R Notebook

library(tidyverse)
library(cowplot)
library(lubridate)
library(mgcv)
source("UVP_2017_library.R")
theme_set(theme_cowplot())
cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')

Particles Only

Read In Data

bes<- read_csv("dataOut/binned_EachSize.csv")

Parsed with column specification:
cols(
  .default = col_double(),
  project = col_character(),
  profile = col_character(),
  time = col_datetime(format = "")
)
See spec(...) for full column specifications.

bds <- read_csv("dataOut/binned_DepthSummary.csv")

Parsed with column specification:
cols(
  .default = col_double(),
  project = col_character(),
  profile = col_character(),
  time = col_datetime(format = "")
)
See spec(...) for full column specifications.

ues <- read_csv("dataOut/unbinned_EachSize.csv")

Parsed with column specification:
cols(
  project = col_character(),
  profile = col_character(),
  time = col_datetime(format = ""),
  depth = col_double(),
  psd_gam = col_double(),
  vol = col_double(),
  sizeclass = col_character(),
  lb = col_double(),
  ub = col_double(),
  binsize = col_double(),
  TotalParticles = col_double(),
  nparticles = col_double(),
  n_nparticles = col_double(),
  biovolume = col_double(),
  speed = col_double(),
  flux = col_double(),
  flux_fit = col_double(),
  GamPredictTP = col_double()
)

uds <- read_csv("dataOut/unbinned_DepthSummary.csv")

Parsed with column specification:
cols(
  .default = col_double(),
  project = col_character(),
  profile = col_character(),
  time = col_datetime(format = "")
)
See spec(...) for full column specifications.

PhoticBase <- 160
OMZBase <- 850

Summary Statistics

PlotNParticles <- uds %>% 
  ggplot(aes(x = tot_nparticles, y = depth, col = profile)) +
 facet_wrap(~project) +
 geom_point(alpha = 0.3, shape = 1) +
scale_y_reverse() + scale_x_log10()

PlotNParticles

bdsAddTime <- bds %>%
  mutate(Hour = hour(time), Day = day(time))

FSG1 <- gam(tot_nparticles~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG2 <- gam(tot_nparticles ~ s(depth, k = 3) + s(Day, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG3 <- gam(tot_nparticles ~ s(depth, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

#FSG4 <- gam(tot_nparticles~ s(depth, k = 3)  + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

summary(FSG1)

Family: gaussian 
Link function: identity 

Formula:
tot_nparticles ~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, 
    bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept)   9.0025     0.1091   82.55   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
            edf Ref.df     F p-value  
s(depth) 1.1031  1.196 3.873  0.0583 .
s(Day)   1.5509  1.796 3.715  0.0801 .
s(Hour)  0.4267  2.000 0.281  0.2713  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.134   Deviance explained = 17.9%
GCV = 0.76575  Scale est. = 0.71367   n = 60

#summary(FSG2)
#summary(FSG3)
#summary(FSG4)

summary(FSG1)$r.sq - summary(FSG2)$r.sq

[1] 0.006778976

summary(FSG2)$r.sq - summary(FSG3)$r.sq

[1] 0.07324885

summary(FSG3)$r.sq

[1] 0.0542546

But there is between projects:

ProjGam <- gam(tot_nparticles~ s(depth, k = 3) + factor(project), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500))

summary(ProjGam)

Family: gaussian 
Link function: identity 

Formula:
tot_nparticles ~ s(depth, k = 3) + factor(project)

Parametric coefficients:
                   Estimate Std. Error t value Pr(>|t|)    
(Intercept)          8.9983     0.4445   20.24   <2e-16 ***
factor(project)P16  17.0001     1.3754   12.36   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
         edf Ref.df     F p-value  
s(depth)   1      1 3.552   0.064 .
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =    0.7   Deviance explained = 70.9%
GCV = 12.411  Scale est. = 11.855    n = 67

library(scales)
#https://stackoverflow.com/questions/30179442/plotting-minor-breaks-on-a-log-scale-with-ggplot
log_breaks = function(maj, radix=10) {
  function(x) {
    minx         = floor(min(logb(x,radix), na.rm=T)) - 1
    maxx         = ceiling(max(logb(x,radix), na.rm=T)) + 1
    n_major      = maxx - minx + 1
    major_breaks = seq(minx, maxx, by=1)
    if (maj) {
      breaks = major_breaks
    } else {
      steps = logb(1:(radix-1),radix)
      breaks = rep(steps, times=n_major) +
               rep(major_breaks, each=radix-1)
    }
    radix^breaks
  }
}
scale_x_log_eng = function(..., radix=10) {
  scale_x_continuous(...,
                     trans=log_trans(radix),
                     breaks=log_breaks(TRUE, radix),
                     minor_breaks=log_breaks(FALSE, radix))
}

#theme_set(theme_bw)
PlotPSDmany <- uds %>% 
  filter(project == "ETNP") %>%
  ggplot(aes(x = psd, y = depth, shape = factor(day(time)), fill = hour(time))) +
 
  #geom_path(aes(x = psd_gam)) + 
  #geom_ribbon(aes(x = psd_gam, xmin = psd_gam - 2 * psd_seg, xmax = psd_gam + 2 * psd_seg), alpha = 0.1, outline_type = "lower") +
  geom_point(alpha = .6, size = 2, stroke = 1) +
  scale_y_reverse(limits = c(1200, 0)) + scale_shape_manual(values = c(21:25)) +
  scale_fill_gradientn(breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  labs(y = "Depth (m)", x = "Particle Size Distribution Slope") + 
  geom_hline(yintercept = 175, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") 

#theme_set(theme_cowplot)

PlotParticlesmany <- uds %>% 
  filter(project == "ETNP") %>%
  ggplot(aes(x = tot_nparticles, y = depth, shape = factor(day(time)), fill = hour(time))) +
 
  #geom_path(aes(x = psd_gam)) + 
  #geom_ribbon(aes(x = psd_gam, xmin = psd_gam - 2 * psd_seg, xmax = psd_gam + 2 * psd_seg), alpha = 0.1, outline_type = "lower") +
  geom_point(alpha = .6, size = 2, stroke = 1) +
  scale_y_reverse(limits = c(1200, 0)) + scale_shape_manual(values = c(21:25)) +
  scale_fill_gradientn(breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  scale_x_log10(breaks = c(10, 100, 1000), minor = c(5, 50, 500)) +
  #theme(legend.position = "none") +
  #scale_x_log_eng()+
  labs(y = "Depth (m)", x = "Particles / L") + 
  geom_hline(yintercept = 175, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") 

# PlotFluxmany <- uds %>% 
#   filter(project == "ETNP") %>%
#   ggplot(aes(x = tot_flux_fit, y = depth, shape = factor(day(time)), fill = hour(time))) +
#  
#   #geom_path(aes(x = psd_gam)) + 
#   #geom_ribbon(aes(x = psd_gam, xmin = psd_gam - 2 * psd_seg, xmax = psd_gam + 2 * psd_seg), alpha = 0.1, outline_type = "lower") +
#   geom_point(alpha = .6, size = 2, stroke = 1) +
#   scale_y_reverse() + scale_shape_manual(values = c(21:25)) +
#   scale_fill_gradientn(breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
#   scale_x_log10() + theme(legend.position = "none")



plot_grid(
  PlotParticlesmany,
  PlotPSDmany,
  rel_widths = c(2, 3)
  )

Removed 266 rows containing missing values (geom_point).Removed 266 rows containing missing values (geom_point).

ggsave("figures/ParticlesPSDMany.png")

Saving 12 x 7.41 in image

ggsave("figures/ParticlesPSDMany.svg")

Saving 12 x 7.41 in image

bdsAddTime <- bds %>%
  mutate(Hour = hour(time), Day = day(time))

FSG1 <- gam(psd~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG2 <- gam(psd ~ s(depth, k = 3) + s(Day, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG3 <- gam(psd ~ s(depth, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG4 <- gam(psd~ s(depth, k = 3)  + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

summary(FSG1)

Family: gaussian 
Link function: identity 

Formula:
psd ~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept) -3.96083    0.01975  -200.6   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df      F  p-value    
s(depth) 1.719  1.921 56.605 3.83e-15 ***
s(Day)   1.000  1.000  1.278   0.2631    
s(Hour)  1.547  2.000  2.763   0.0347 *  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.639   Deviance explained = 66.5%
GCV = 0.025652  Scale est. = 0.023401  n = 60

#summary(FSG2)
#summary(FSG3)
summary(FSG4)

Family: gaussian 
Link function: identity 

Formula:
psd ~ s(depth, k = 3) + s(Hour, k = 4, bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept) -3.96083    0.01978  -200.3   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df      F  p-value    
s(depth) 1.721  1.922 56.019 4.39e-15 ***
s(Hour)  1.609  2.000  3.023   0.0294 *  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.638   Deviance explained = 65.9%
GCV = 0.025297  Scale est. = 0.023471  n = 60

summary(FSG1)$r.sq - summary(FSG2)$r.sq

[1] 0.03439091

summary(FSG2)$r.sq - summary(FSG3)$r.sq

[1] 0.003377312

summary(FSG3)$r.sq

[1] 0.6015425

Not a significant difference in PSD with respect to time.

But there is between projects:

ProjGam <- gam(psd~ s(depth, k = 3) + factor(project), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500))

summary(ProjGam)

Family: gaussian 
Link function: identity 

Formula:
psd ~ s(depth, k = 3) + factor(project)

Parametric coefficients:
                   Estimate Std. Error  t value Pr(>|t|)    
(Intercept)        -3.96167    0.02491 -159.029   <2e-16 ***
factor(project)P16 -0.19977    0.07708   -2.592   0.0118 *  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F  p-value    
s(depth) 1.224  1.398 34.54 2.26e-08 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.437   Deviance explained = 45.6%
GCV = 0.039117  Scale est. = 0.037234  n = 67

I wonder if I can show that the profiles aren’t statistically significanlty different. Or that they are for that matter… I think in that case, I run a gam with and without a parameter for profile… And then quantify the effect size of that parameter

Or follow this Gavin Simpson Post https://fromthebottomoftheheap.net/2017/10/10/difference-splines-i/

or anova.gam {mgcv}

Calculate gams for each profile, and then run anova.gam to see if they are different…

PlotNParticlesEP <- uds %>% 
  filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(x = tot_nparticles, y = depth, col = project, shape = project)) +
 geom_point(alpha = 0.7, size = 2, stroke = 1) +
  #geom_path(aes(x = tot_nparticles)) +
  #geom_ribbon(aes(x = psd_gam, xmin = psd_gam - 2 * psd_seg, xmax = psd_gam + 2 * psd_seg), alpha = 0.1) +
scale_y_reverse(limits = c(1000, 0)) + scale_x_log10() + scale_color_manual(values = c("gray20", "brown")) +
  labs(x = "Particles/L", y = "Depth (m)") +
  theme(legend.position = "none") +
  scale_shape_manual(values = c(1:5)) +
  geom_hline(yintercept = 175, color = "darkgreen") +
  geom_hline(yintercept = 200, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") 

PlotNParticlesEP

I removed one outlyer from p16 for visualization purposes (300 particles/l at surface)

PlotPSDEP <- uds %>% 
  filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(x = psd, y = depth, col = project, shape = project)) +
 geom_point(alpha = 0.7, size = 2, stroke = 1) +
  geom_path(aes(x = psd_gam)) +
  geom_ribbon(aes(x = psd_gam, xmin = psd_gam - 2 * psd_seg, xmax = psd_gam + 2 * psd_seg), alpha = 0.1) +
scale_y_reverse(limits = c(1000, 0)) + scale_color_manual(values = c("gray20", "brown"))  +
  scale_shape_manual(values = c(1:5)) + labs(y = "", x = "Particle Size Distribution Slope") +
  geom_hline(yintercept = 175, color = "darkgreen") +
  geom_hline(yintercept = 200, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") 

PlotPSDEP

I may just cow these togther.

plot_grid(PlotNParticlesEP, PlotPSDEP, rel_widths = c(2,3), labels = c("A", "B"))

Removed 1211 rows containing missing values (geom_point).Removed 1211 rows containing missing values (geom_point).Removed 1211 row(s) containing missing values (geom_path).

ggsave("figures/ParticlesAndPSD_ETNPVsP16.svg")

Saving 10 x 4 in image

ggsave("figures/ParticlesAndPSD_ETNPVsP16.png")

Saving 10 x 4 in image

mainParticleComponents <- bds %>%
  filter(profile %in% c("stn_043", "p16n_100")) %>%
  select(project, profile, depth,
         tot_nparticles, small_nparticles, big_nparticles,
         tot_psd = psd, small_psd, big_psd,
         tot_flux_fit, small_flux_fit, big_flux_fit) %>%
  pivot_longer(cols = -c("project", "profile", "depth")) %>%
  separate(name, c("size", "meas")) %>%
  mutate(meas = recode(meas, nparticles = "particles/L")) %>%
  mutate(meas = factor(meas, levels = c("particles/L", "flux", "psd")))

Expected 2 pieces. Additional pieces discarded in 273 rows [7, 8, 9, 16, 17, 18, 25, 26, 27, 34, 35, 36, 43, 44, 45, 52, 53, 54, 61, 62, ...].

PlotFlx <- mainParticleComponents %>% 
  filter(meas != "psd") %>%
  ggplot(aes(y = depth, x = value, col = project, shape = project)) + facet_grid(size ~ meas, scales = "free_x") + geom_point(size = 2) + scale_y_reverse(limits = c(1000, 0)) + scale_x_log10() + theme(axis.title.x = element_blank(), legend.position = "none", strip.background.y = element_blank(), strip.text.y = element_blank(), plot.margin = unit(c(7,0,7,7), "pt")) + scale_color_manual(values = c("brown", "gray20")) + scale_shape_manual(values = c(1:5)) + theme(axis.text.x = element_text(angle = 90)) + geom_hline(yintercept = 175, color = "darkgreen")

PlotPSD <- mainParticleComponents %>% 
  filter(meas == "psd") %>%
  ggplot(aes(y = depth, x = value, col = project, shape = project)) + facet_grid(size~meas, scales = "free_x") + geom_point(size = 2) + scale_y_reverse(limits = c(1000, 0)) +
  theme(axis.title.x = element_blank(), axis.title.y = element_blank(), axis.line.y = element_blank(), axis.text.y = element_blank(), axis.ticks.y = element_blank(), plot.margin = unit(c(7,7,26.5,0), "pt")) +
  scale_color_manual(values = c("brown", "gray20")) +  scale_shape_manual(values = c(1:5)) +  theme(axis.text.x = element_text(angle = 90)) + geom_hline(yintercept = 175, color = "darkgreen")

plot_grid(PlotFlx, PlotPSD, rel_widths = c(3, 2))

Removed 246 rows containing missing values (geom_point).Removed 123 rows containing missing values (geom_point).

ggsave("figures/BigVsSmall.svg")

Saving 7.29 x 4.5 in image

ggsave("figures/BigVsSmall.png")

Saving 7.29 x 4.5 in image

Flux small and flux tot track so closely because ag > psd. since the size distribution of the flux sould be PSD + ag (psd is negative in this case). Yo ucan see the variance at the one depth where psd is flatest at the very top. time 2017-01-13 17:51:31 202.4 L

eg_dataline <- bds %>% 
  filter(profile == "stn_043", depth == 162.5)
eg_slope =  eg_dataline %>% pull(psd)
eg_icp = eg_dataline %>% pull(icp)
eg_vol = eg_dataline %>% pull(vol)

eg_datablock <- bes %>%
  filter(profile == "stn_043", depth == 162.5)


eg_lb = eg_datablock$lb
eg_binsize = eg_datablock$binsize
eg_nnp = exp(eg_icp + log(eg_lb) * eg_slope)

eg_np = eg_nnp * eg_binsize
eg_tp = eg_np * eg_vol
eg_df <- tibble(lb = eg_lb, n_nparticles = eg_nnp, nparticles = eg_np, TotalParticles = eg_tp)


EgNNP <- eg_datablock %>%
  ggplot(aes(x = lb, y = n_nparticles)) + geom_point() + scale_x_log10() + scale_y_log10() + 
  geom_path(data = eg_df) + labs(y = "Binsize & Volume Normalized \n Particles (#/L/mm)", x = "Size (mm)")

EgNP <- eg_datablock %>%
  ggplot(aes(x = lb, y = nparticles)) + geom_point() + scale_x_log10() + scale_y_log10() + 
  geom_path(data = eg_df) + labs(y = "Normalized Particles" , x = "Size (mm)")

EgTP <- eg_datablock %>%
  ggplot(aes(x = lb, y = TotalParticles)) + geom_point() + scale_x_log10() + scale_y_log10() + 
  geom_path(data = eg_df) + labs( y = "Total Particles Observed (#)", x = "Size (mm)")

plot_grid(EgNNP, EgTP, labels = c("A", "B"))

Transformation introduced infinite values in continuous y-axisTransformation introduced infinite values in continuous y-axis

ggsave("figures/ExamplePSD163m.png")

Saving 7.29 x 4.5 in image

ggsave("figures/ExamplePSD163m.svg")

Saving 7.29 x 4.5 in image

Smooth flux and um disaggregation.

bds %>% 
  ggplot(aes(y = depth, x = Flux_Smooth, col = factor(time))) + facet_wrap(~project) + geom_point() + scale_y_reverse(limits = c(1000, 0)) + scale_x_log10()

bdsAddTime <- bds %>%
  mutate(Hour = hour(time), Day = day(time))

FSG1 <- gam(Flux_Smooth~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG2 <- gam(Flux_Smooth ~ s(depth, k = 3) + s(Day, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

FSG3 <- gam(Flux_Smooth ~ s(depth, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

summary(FSG1)

Family: gaussian 
Link function: identity 

Formula:
Flux_Smooth ~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, 
    bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept)  34.7319     0.9312    37.3   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df      F  p-value    
s(depth) 1.874  1.984 12.034 0.000111 ***
s(Day)   1.828  1.969  2.774 0.089781 .  
s(Hour)  1.515  2.000  2.784 0.031360 *  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.333   Deviance explained = 39.2%
GCV = 58.038  Scale est. = 52.024    n = 60

summary(FSG2)

Family: gaussian 
Link function: identity 

Formula:
Flux_Smooth ~ s(depth, k = 3) + s(Day, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept)  34.7319     0.9774   35.53   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df      F  p-value    
s(depth) 1.864  1.981 10.835 0.000255 ***
s(Day)   1.737  1.931  1.586 0.243284    
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.265   Deviance explained =   31%
GCV = 62.081  Scale est. = 57.32     n = 60

summary(FSG3)

Family: gaussian 
Link function: identity 

Formula:
Flux_Smooth ~ s(depth, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept)  34.7319     0.9944   34.93   <2e-16 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F  p-value    
s(depth) 1.857  1.979 10.73 0.000277 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.239   Deviance explained = 26.3%
GCV = 62.291  Scale est. = 59.325    n = 60

summary(FSG1)$r.sq - summary(FSG2)$r.sq

[1] 0.06791706

summary(FSG2)$r.sq - summary(FSG3)$r.sq

[1] 0.02571191

summary(FSG3)$r.sq

[1] 0.2392424

bds %>% filter(project == "ETNP") %>% select(profile, depth, Flux_Smooth) %>% pivot_wider(names_from = profile, values_from = Flux_Smooth)

Something is off. All of the flux profiles are identical. Skip this

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
plt1 <- bds %>% #filter(DFP > 1) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = DFP, col = factor(time), shape = factor(time))) + facet_wrap(~project) + geom_point() + scale_y_reverse(limits = c(1000, 0)) + xlim(c(0.5, 1.5))+ geom_vline(xintercept = 1) +
   scale_color_manual(values = c(rep("black", 5), rep("blue", 5))) + scale_shape_manual(values = rep(1:5, 2))

plotly::ggplotly(plt1)

What the heck is going on with DFP here. Why is it usually > 1 shouldn’t it be less than 1 when flux is decreasing? This very deep increasing flux seems improbable to me. Lets check the smooths. Or only go to 1000m.

, legend.background = element_blank(), legend.box.background = element_rect()

scientific_10 <- function(x) {parse(text=gsub("e\\+*", " %*% 10^", scales::scientific_format()(x))) }
scientific_10_b <- function(x) {parse(text=gsub("e\\+*", " %*% 10^", scales::scientific_format()(x))) }

scientific_10_c <- function(x) {
    xout <- gsub("1e", "10^{", format(x),fixed=TRUE)
    xout <- gsub("{-0", "{-", xout,fixed=TRUE)
    xout <- gsub("{+", "{", xout,fixed=TRUE)
    xout <- gsub("{0", "{", xout,fixed=TRUE)
    xout <- paste(xout,"}",sep="")
    return(parse(text=xout))
    
}

scale_x_log10nice <- function(name=NULL,omag=seq(-10,20),...) {
    breaks10 <- 10^omag
    scale_x_log10(breaks=breaks10,labels=scientific_10_c(breaks10),...)
}


#https://stackoverflow.com/questions/10762287/how-can-i-format-axis-labels-with-exponents-with-ggplot2-and-scales
#jacob_magnitude <- function(x){expression(10^round(log10(x)))}

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
pltFlx <- bds %>% filter(project == "ETNP") %>% #filter(DFP > 1) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = Flux_Smooth, shape = factor(day(time)), fill = hour(time), group = factor(time)))  + geom_point(size = 2, stroke = 1)+
  #geom_path() +
  scale_y_reverse(limits = c(1000, 0))+
  scale_x_log10nice()+
  #scale_x_log10() + 
   scale_color_gradient2(low = "darkgreen", mid = "gray80", high = "purple", midpoint = 10) + scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) + 
  scale_fill_gradientn(name = "Hour of Day", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  
  labs(x = bquote(Smoothed~Flux~(µmol~C/m^2/d)), y = "Depth (m)") +
  #labs(x = "moo", y = "Depth (m)") +
  geom_rect(data = data.frame(project = "ETNP"), aes(xmin = 20, xmax = 180, ymin = 75, ymax = 500), colour = "red", fill = NA, inherit.aes = FALSE) +
  theme(axis.text.x = element_text(angle = 90, vjust = .3), legend.spacing = unit(.1, "cm")) +
   geom_segment(aes(y = 160, yend = 160, x = 20, xend = 500), color = "darkgreen", stroke = 0.5)+
   geom_segment(aes(y = 850, yend = 850, x = 20, xend = 500), color = "darkblue", stroke = 0.5)#+ geom_hline(yintercept = 850, color = "darkblue")

Ignoring unknown parameters: strokeIgnoring unknown parameters: stroke

pltFlxNoLegend <- pltFlx + theme(legend.position = "none")
pltFlxLegend <- get_legend(pltFlx)

Removed 14 rows containing missing values (geom_point).

pltFlx

#plotly::ggplotly(plt1)

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
pltFlxZoom <- bds %>% filter(project == "ETNP" & depth <= 500 & depth >= 75) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = Flux_Smooth, shape = factor(day(time)), fill = hour(time), group = factor(time))) + geom_point(size = 2, stroke = 1)+
  #geom_path() +
  scale_y_reverse()+
  #scale_x_log10() +
  scale_x_log10(breaks = c(seq(from = 20, to = 50, by = 10), seq(from = 60, to = 180, by = 20)), limits = c(20, 180)) +
   scale_color_gradient2(low = "darkgreen", mid = "gray80", high = "purple", midpoint = 10) + scale_shape_manual(values = rep(21:25, 2)) + 
  scale_fill_gradientn(breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  theme(axis.text.x = element_text(angle = 90)) +
labs(x = "Smoothed Flux", y = "Depth") + theme(legend.position = "none")+
geom_hline(yintercept = 160, color = "darkgreen")

pltFlxZoom

#plotly::ggplotly(plt1)

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
pltDelta3 <- bds %>% filter(project == "ETNP") %>% #filter(DFP > 1) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = pracma::nthroot(DF/DZ, 5), shape = factor(day(time)), fill = hour(time), group = factor(time)))  + geom_point(size = 2, stroke = 1)+
  #geom_path() +
  scale_y_reverse(limits = c(1000, 0))+
  scale_x_continuous(limits = c(-2.1, .6), breaks = seq(from = -2, to = .75, by = 0.5)) +
  #scale_x_log10() +
   scale_color_gradient2(low = "darkgreen", mid = "gray80", high = "purple", midpoint = 10) + scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) + 
  scale_fill_gradientn(name = "Hour", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  geom_vline(xintercept = 0) +
  labs(x = bquote((DF/DZ)^{1/5}~(µmolC/m^3/d)^{1/5}), y = "Depth (m)") + theme(legend.pos = "none")+
  geom_hline(yintercept = 160, color = "darkgreen") + geom_hline(yintercept = 850, color = "darkblue")
  #labs(x = "(DF/DZ) ^ 1/5 (µmol C/m^3/d) ^ 1/5")

pltDelta3

#plotly::ggplotly(plt1pos)

# #plot_grid(pltFlxNoLegend, pltFlxZoom, pltDelta3, pltFlxLegend)
# 
# pltFlxLegend <- get_legend(pltFlx + theme(legend.box.margin = margin(0, 0, 40, 10)))
# 
# pgTop <- plot_grid(pltFlxNoLegend, pltFlxZoom + theme(plot.margin = unit(c(1, 0, 3, 0), units = "cm")), rel_widths = c(2, 1), labels = c("A", "B"))
# pgBottom <- plot_grid(pltDelta3, pltFlxLegend , rel_widths = c(3, 1), labels = c("C", ""), label_size = 14)
# pgBoth <- plot_grid(pgTop, pgBottom, ncol = 1)
# 
# pgBoth
# 
# ggsave("figures/FluxDeepDive.png")
# ggsave("figures/FluxDeepDive.svg")

Within panel drawing

pgTop <- ggdraw(pltFlxNoLegend 
       ) +
  draw_plot(pltFlxZoom, .4, .25, .55, .60) +
  draw_plot_label(
    c("","B"),
    c(.05, 0.55),
    c(1, 0.85),
    size = 16
  )

Removed 14 rows containing missing values (geom_point).

pgTop

pgBottom <- plot_grid(pltDelta3, pltFlxLegend , rel_widths = c(3, 1), labels = c(“C”, ""), label_size = 14)

I don’t know whats going on below here

pgBottom <- pltDelta3  + geom_rect(aes(xmin = -2, xmax = -1.15, ymin = 170, ymax = 1000), colour = "gray50", fill = NA, inherit.aes = FALSE) + draw_plot(pltFlxLegend , -1.9, -575, .7)
pgBoth <- plot_grid(pgTop + theme(plot.margin = unit(c(0, 0, 0, 0), units = "cm")),
                    pgBottom + theme(plot.margin = unit(c(0, 0, 0, 0), units = "cm")),
                    ncol = 1, rel_heights = c(4, 4), labels = c("A", "C"), label_size = 16)

Removed 33 rows containing missing values (geom_point).

pgBoth

ggsave("figures/FluxDeepDive.png")

Saving 5 x 9 in image

ggsave("figures/FluxDeepDive.svg")

Saving 5 x 9 in image

# #plot_grid(pltFlxNoLegend, pltFlxZoom, pltDelta3, pltFlxLegend)
# 
# pltFlxLegend <- get_legend(pltFlx + theme(legend.box.margin = margin(0, 0, 40, 10)))
# 
# pgTop <- plot_grid(pltFlxNoLegend + ylim(c(1000, 0)), pltFlxZoom + theme(plot.margin = unit(c(1, 0, 3, 0), units = "cm")), rel_widths = c(2, 1), labels = c("A", "B"))
# pgBottom <- plot_grid(pltDelta3 + ylim(c(1000, 0)), pltFlxLegend , rel_widths = c(3, 1), labels = c("C", ""))
# pgBoth <- plot_grid(pgTop, pgBottom, ncol = 1)
# 
# pgBoth
# 
# #ggsave("figures/FluxShallowDive.png")
# #ggsave("figures/FluxShallowDive.svg")

Test for day to day and hourly variability in delta flux

bdsAddTime <- bds %>% 
  mutate(Hour = hour(time), Day = day(time))

DFG1 <- gam(pracma::nthroot(DF/DZ, 5)~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 250 & depth <=500 & project == "ETNP"))

DFG2 <- gam(pracma::nthroot(DF/DZ, 5) ~ s(depth, k = 3) + s(Day, k = 3), data = bdsAddTime %>% filter(depth >= 250 & depth <=500 & project == "ETNP"))

DFG3 <- gam(pracma::nthroot(DF/DZ, 5) ~ s(depth, k = 3), data = bdsAddTime %>% filter(depth >= 250 & depth <=500 & project == "ETNP"))

summary(DFG1)

Family: gaussian 
Link function: identity 

Formula:
pracma::nthroot(DF/DZ, 5) ~ s(depth, k = 3) + s(Day, k = 3) + 
    s(Hour, k = 4, bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)   
(Intercept) -0.18385    0.05697  -3.227  0.00267 **
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F p-value   
s(depth) 1.716  1.919 4.652 0.03562 * 
s(Day)   1.931  1.995 6.788 0.00412 **
s(Hour)  1.555  2.000 3.247 0.02318 * 
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.328   Deviance explained = 41.3%
GCV = 0.15991  Scale est. = 0.1363    n = 42

summary(DFG2)

Family: gaussian 
Link function: identity 

Formula:
pracma::nthroot(DF/DZ, 5) ~ s(depth, k = 3) + s(Day, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)   
(Intercept) -0.18385    0.06186  -2.972  0.00514 **
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F p-value  
s(depth) 1.641  1.871 3.599  0.0798 .
s(Day)   1.827  1.970 3.034  0.0463 *
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.207   Deviance explained = 27.4%
GCV = 0.17984  Scale est. = 0.1607    n = 42

summary(DFG3)

Family: gaussian 
Link function: identity 

Formula:
pracma::nthroot(DF/DZ, 5) ~ s(depth, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)   
(Intercept) -0.18385    0.06635  -2.771  0.00849 **
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F p-value
s(depth) 1.592  1.834 3.213   0.107

R-sq.(adj) =  0.0878   Deviance explained = 12.3%
GCV = 0.19707  Scale est. = 0.18491   n = 42

summary(DFG1)$r.sq - summary(DFG2)$r.sq

[1] 0.1204131

summary(DFG2)$r.sq - summary(DFG3)$r.sq

[1] 0.1193917

summary(DFG3)$r.sq

[1] 0.08782536

png(filename = “./figures/CombinedP2Info.png”, width = 10, height = 8, units = “in”, res = 200) StationInfoPlot() dev.off()

#plot.new()
FluxGamPlot <- function(){
  par(mfrow = c(2,2))
  plot(DFG1)
  mtext(expression(bold("C")), side = 3, line = 0, adj = 0, cex = 2)
  par(mfg = c(1,1))
  mtext(expression(bold("A")), side = 3, line = 0, adj = 0, cex = 2)
  par(mfg = c(1,2))
  mtext(expression(bold("B")), side = 3, line = 0, adj = 0, cex = 2)
}

FluxGamPlot()

png(filename = "./figures/FluxGamPlot.png", width = 10, height = 8, units = "in", res = 200)

FluxGamPlot()
dev.off()

png 
  2 

Check of actual data for hour

ggplot(data = bds %>% filter(depth >= 175, depth <= 500), aes(y = DF/DZ, x = hour(time), col = depth, group = depth)) + geom_point() + geom_line()

#Osps

(u mol C / m^3 / day)

disagFig <- bds %>% filter(project == "ETNP") %>%
  ggplot(aes(y = depth, x = pracma::nthroot(ospsDZ, 3), shape = factor(day(time)), fill = hour(time), group = factor(time))) + geom_point(size = 2) + scale_y_reverse(limits = c(1000, 0)) +
  scale_x_continuous(limits = c(-1, 1)) +
  geom_vline(xintercept = 0) +   scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) +
  #labs(x = bquote("Observed - Modeled Small Particle Flux"~(μmol/m^3/day)), y = "Depth (m)") +
  labs(x = paste("OSMS", expression((μmol/m^3/day)))) +
  scale_fill_gradientn(name = "Hour of Day", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) + geom_hline(yintercept = 175, color = "darkgreen") + geom_hline(yintercept = 850, color = "darkblue")
disagFig

#ggsave("..figures/FluxSizeShift.svg"

 ggsave("figures/FluxSizeShift.png")

Saving 6 x 4 in image

 ggsave("figures/FluxSizeShift.svg")

Saving 6 x 4 in image

Clara Fig

For NSF Proposal plot_grid( PlotParticlesmany, PlotPSDmany, rel_widths = c(2, 3) )

pgTop <- ggdraw(pltFlxNoLegend 
       ) +
  draw_plot(pltFlxZoom, .4, .25, .55, .60) +
  draw_plot_label(
    c("","D"),
    c(.05, 0.55),
    c(1, 0.85),
    size = 16
  )

Removed 14 rows containing missing values (geom_point).

pgCF <- plot_grid(PlotParticlesmany + ylim(c(1000, 0)),
                  PlotPSDmany + ylim(c(1000, 0)) +theme(legend.position = "none"),
                  pgTop + theme(plot.margin = unit(c(0, 0, 0, 0), units = "cm")),
                    disagFig + theme(plot.margin = unit(c(0, 0, .2, 0), units = "cm")),
                    ncol = 2, rel_heights = c(4, 4), labels = c("A", "B", "C", "E"), label_size = 16)

Scale for 'y' is already present. Adding another scale for 'y', which will replace the existing scale.
Scale for 'y' is already present. Adding another scale for 'y', which will replace the existing scale.
Removed 308 rows containing missing values (geom_point).Removed 308 rows containing missing values (geom_point).Removed 37 rows containing missing values (geom_point).

pgCF

ggsave("./figures/UVP_for_zoop_proposal.png", pgCF, width = 10, height = 8, units = "in")

bdsAddTime <- bds %>%
  mutate(Hour = hour(time), Day = day(time))

OZG1 <- gam(ospsDZ ~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc"), knots = list(Hour = c(0, 24)), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

OZG2 <- gam(ospsDZ ~ s(depth, k = 3) + s(Day, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

OZG3 <- gam(ospsDZ ~ s(depth, k = 3), data = bdsAddTime %>% filter(depth >= 175 & depth <=500 & project == "ETNP"))

summary(OZG1)

Family: gaussian 
Link function: identity 

Formula:
ospsDZ ~ s(depth, k = 3) + s(Day, k = 3) + s(Hour, k = 4, bs = "cc")

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept) 0.023796   0.002244    10.6 6.42e-15 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
            edf Ref.df     F  p-value    
s(depth) 1.9032  1.991 8.166 0.000545 ***
s(Day)   1.8673  1.982 4.932 0.015920 *  
s(Hour)  0.1508  2.000 0.080 0.345843    
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.304   Deviance explained = 35.1%
GCV = 0.00032912  Scale est. = 0.00030213  n = 60

summary(OZG2)

Family: gaussian 
Link function: identity 

Formula:
ospsDZ ~ s(depth, k = 3) + s(Day, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept) 0.023796   0.002247   10.59 6.54e-15 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F  p-value    
s(depth) 1.904  1.991 8.157 0.000549 ***
s(Day)   1.868  1.983 4.868 0.016638 *  
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.302   Deviance explained = 34.7%
GCV = 0.00032916  Scale est. = 0.00030298  n = 60

summary(OZG3)

Family: gaussian 
Link function: identity 

Formula:
ospsDZ ~ s(depth, k = 3)

Parametric coefficients:
            Estimate Std. Error t value Pr(>|t|)    
(Intercept)   0.0238     0.0024   9.916 5.03e-14 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Approximate significance of smooth terms:
           edf Ref.df     F  p-value    
s(depth) 1.894  1.989 7.458 0.000924 ***
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

R-sq.(adj) =  0.204   Deviance explained =   23%
GCV = 0.00036308  Scale est. = 0.00034556  n = 60

summary(OZG1)$r.sq - summary(OZG2)$r.sq

[1] 0.001957736

summary(OZG2)$r.sq - summary(OZG3)$r.sq

[1] 0.09804936

summary(OZG3)$r.sq

[1] 0.2043621

OSMSGamPlot <- function(){
  par(mfrow = c(1,2))
  plot(OZG2)
  mtext(expression(bold("B")), side = 3, line = 0, adj = 0, cex = 2)
  par(mfg = c(1,1))
  mtext(expression(bold("A")), side = 3, line = 0, adj = 0, cex = 2)
}

OSMSGamPlot()

png(filename = "./figures/OSMSGamPlot.png", width = 10, height = 6, units = "in", res = 200)

OSMSGamPlot()

dev.off()

png 
  2 

plot(OZG2)

bds %>% filter(project == "P16") %>%
  ggplot(aes(y = depth, x = ospsDZ)) + facet_wrap(~project) + geom_point() + scale_y_reverse(limits = c(500, 0)) + geom_vline(xintercept = 0)

Trap data

trapFlux3 <- read_csv("dataOut/fluxMS_distilled.csv")

Parsed with column specification:
cols(
  Class = col_character(),
  Depth = col_double(),
  TrapID = col_character(),
  TrapType = col_character(),
  SampleType = col_character(),
  C_flux = col_double(),
  C_flux_umol = col_double()
)

UVPFluxComb <- read_csv("dataOut/CombinedProfileFluxEst_DS.csv")

Parsed with column specification:
cols(
  depth = col_double(),
  Flux = col_double()
)

UVPFluxOE <- read_csv("dataOut/ObservedVsExpectedFlux.csv")

Parsed with column specification:
cols(
  depth = col_double(),
  tn_flux = col_double(),
  profile = col_character(),
  project = col_character(),
  time = col_character(),
  tot_flux2 = col_double()
)

trapFlux3

UVPFluxComb

fluxMS_distilled_toPlot <- trapFlux3 %>%
  mutate(SampleType = recode(SampleType, `plus.p` = "plus-particles", top = "top-collector"))

Remove traps where mass spec didn’t work correctly 2-17 150 1-12 73m 1-12 148 2-14 100 |(TrapID == “2-17” & Depth == 150)

fluxMS_distilled_toPlot2 <- fluxMS_distilled_toPlot %>%
 filter(!((TrapID == "1-12") | (TrapID == "2-14" & Depth == 100)|(TrapID == "2-17" & Depth == 150)))
fluxMS_distilled_toPlot2

UVPFluxComb %>%
  ggplot(aes(y = depth))  + scale_y_reverse(limits = c(1000, 0)) +
  scale_x_continuous(limits = c(0, 200)) +
  geom_point(aes(y = Depth, x = C_flux_umol, fill = SampleType, shape = TrapType),
             colour = "black", stroke = 1, size = 5, data = fluxMS_distilled_toPlot) +
  geom_point(aes(x = Flux), size = 3, shape = 21, color = "white", fill = "black") +
  geom_point(aes(x = -1, y = -1, size = "UVP")) + # dummy point for the legend
scale_shape_manual(values = c(25, 22))+
  scale_size_manual(values = 1, name = "") +
  ylab("Depth (m)") + xlab("Flux µmolC/m^2/day") +
  guides(fill = guide_legend(override.aes = list(shape = 21))) +
  scale_fill_viridis_d() +
  theme_cowplot() + 
  theme(
        legend.position = c(0.5, 0.4),
        legend.box.background = element_rect(color = "black", size = 0.5),
        legend.margin = margin(-10, 5, 10, 5)
  ) +
  geom_rect(data = data.frame(project = "ETNP"), aes(xmin = 15, xmax = 32, ymin = 45, ymax = 195), colour = "red", fill = NA, inherit.aes = FALSE)
 ggsave("figures/FittedFlux.png")

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 ggsave("figures/FittedFlux.svg")

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Traps where mass spec didn’t work.

UVPFluxPlot00 <- UVPFluxComb %>% 
  ggplot(aes(y = depth))  + scale_y_reverse(limits = c(1000, 0)) +
  scale_x_continuous(limits = c(0, 200)) +
  geom_point(aes(y = Depth, x = C_flux_umol, shape = TrapType, ID = TrapID),
             colour = "black", stroke = 1, size = 5, data = fluxMS_distilled_toPlot2) +
  geom_line(aes(x = Flux), size = 1, color = "black") +
  geom_point(aes(x = -1, y = -1, size = "UVP Estimate")) + # dummy point for the legend
  geom_point(aes(x = tot_flux2), size = 3, shape = 21, color = "white", fill = "black", data = UVPFluxOE) +
scale_shape_manual(values = c(25, 22))+
  scale_size_manual(values = 1, name = "") +
  ylab("Depth (m)") +
  #xlab(expression(Flux µmolC/m^2/day)) +
  xlab(expression(paste("x axis ", ring(A)^2))) +
  xlab(expression(paste("Flux (µmolC/", m^2, "/day)"))) +
  
  guides(fill = guide_legend(override.aes = list(shape = 21))) +
  theme_cowplot() + 
  theme(
        legend.position = c(0.5, 0.4),
        legend.box.background = element_rect(color = "black", size = 0.5),
        legend.margin = margin(-10, 5, 10, 5)
  ) 

Ignoring unknown aesthetics: ID

# UVPFluxPlot <- UVPFluxPlot00 +
#   geom_rect(data = data.frame(project = "ETNP"), aes(xmin = 15, xmax = 32, ymin = 45, ymax = 195), colour = "red", fill = NA, inherit.aes = FALSE)

UVPFluxPlot00

ggsave("figures/FittedFlux.png")

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ggsave("figures/FittedFlux.svg")

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Example particle size distribution

horizontalGamPlot <- dataGamHorizontal %>% ggplot(aes(x = resp_fit, y = depth, col = log(lb), group = lb)) + scale_y_reverse() + geom_point() + scale_x_log10(limits = c(10^-8, NA)) + scale_color_viridis_c() + geom_path() + geom_vline(xintercept = 1) + geom_vline(xintercept = 5) + geom_errorbar(aes(xmin = resp_lower, xmax = resp_upper), width = 10, alpha = 0.5)+ theme_bw()

TPPlot <- bes %>% filter(profile == "stn_043") %>% group_by(lb) %>% ggplot(aes(x = TotalParticles, y = depth, col = log(lb), group = lb)) + scale_y_reverse(limits = c(1000, 0)) + geom_point() + scale_x_log10() + scale_color_viridis_c() + geom_path() + geom_vline(xintercept = 1) + geom_vline(xintercept = 5) + labs(y = "Depth (m)", x = "TotalParticles Observed (#)")

nnpPlot <- bes %>% filter(profile == "stn_043") %>% group_by(lb) %>% ggplot(aes(x = n_nparticles, y = depth, col = log(lb), group = lb)) + scale_y_reverse(limits = c(1000, 0)) + geom_point() + scale_x_log10() + scale_color_viridis_c() + geom_path() + geom_vline(xintercept = 1) + geom_vline(xintercept = 5) + labs(y = "Depth (m)", x = "Binsize and Volume Normalized Particles (#/L/mm)")

FitPlot <- bes %>% filter(profile == "stn_043") %>% group_by(lb) %>% ggplot(aes(x = nnp_smooth, xmin = nnp_lower, xmax = nnp_upper, y = depth, col = log(lb), group = lb)) + scale_y_reverse(limits = c(1000, 0)) + geom_point() + scale_x_log10() + scale_color_viridis_c() + geom_path() + geom_vline(xintercept = 1) + geom_vline(xintercept = 5) + labs(y = "Depth (m)", x = "Smoothed - Normalized Particles (#/L/mm)") + geom_errorbar(width = 10, alpha = 0.5)

npLegend <- get_legend(FitPlot + theme(legend.box.margin = margin(0, 0, 40, 200)) + labs(col = expression(log[e](Size (mm)))))

Removed 325 rows containing missing values (geom_point).Removed 325 row(s) containing missing values (geom_path).

plot_grid(
  TPPlot + theme(legend.position = "none"),
  nnpPlot + theme(legend.position = "none"),
  npLegend ,
  FitPlot + theme(legend.position = "none")
)

Transformation introduced infinite values in continuous x-axisTransformation introduced infinite values in continuous x-axisRemoved 325 rows containing missing values (geom_point).Removed 325 row(s) containing missing values (geom_path).Transformation introduced infinite values in continuous x-axisTransformation introduced infinite values in continuous x-axisRemoved 325 rows containing missing values (geom_point).Removed 325 row(s) containing missing values (geom_path).Removed 325 rows containing missing values (geom_point).Removed 325 row(s) containing missing values (geom_path).

ggsave("figures/AllParticleSizes.svg")

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ggsave("figures/AllParticleSizes.png")

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Weber Bianchi Figs

SameGam <- gam(TotalParticles ~s(log(lb), log(depth), by = factor(time)), offset = log(vol * binsize), family = nb(),
    data = bes %>% filter(project == "ETNP"))

besE <- bes %>% filter(project == "ETNP")

lb_new <- exp(seq(from = log(0.1), to = log(2.1), by = 0.05))
ub_new <- lead(lb_new)
binsize_new <- ub_new - lb_new

lbbs <- tibble(lb = lb_new, ub = ub_new, binsize = binsize_new)

Expanded <- expand_grid(lb = exp(seq(from = log(0.1), to = log(2), by = 0.05)), depth = seq(from = 20, to = 2000, by = 20), time = as.factor(unique(besE$time))) %>% left_join(lbbs, by = "lb")

Pred <- exp(predict(SameGam, Expanded))
ToPlot <- bind_cols(Expanded, nnparticles = Pred) %>% mutate(time = as.character(time)) %>% mutate(nparticles = nnparticles * binsize)

ToPlot %>% filter(lb <= 2) %>%  ggplot(aes(x = lb, y = depth, fill = log10(nnparticles), z = log10(nnparticles))) + geom_tile() + scale_fill_viridis_c() + scale_y_reverse() + scale_x_log10()  + facet_wrap(~time) + geom_contour(color = "black")

meanBese <- ToPlot %>% filter(lb <= 2) %>% group_by(lb, depth) %>% summarize(nparticles = mean(nparticles), nnparticles = mean(nnparticles))

WBColorMap <- meanBese%>%
   ggplot(aes(x = lb, y = depth, fill = log10(nnparticles), z = log10(nnparticles))) + geom_tile() + scale_fill_viridis_c(name = "log10(number density \n (normalized))") + scale_y_reverse() + scale_x_log10() + geom_contour(color = "black") + geom_hline(yintercept = 160, color = "darkgreen") + geom_hline(yintercept = 850, color = "darkblue")
WBColorMap

Average of everything

#meanBese043 <- ToPlot %>% filter(lb <= 2, time == "2017-01-13 11:51:31")

meanBese%>%
   ggplot(aes(x = lb, y = depth, fill = log10(nnparticles), z = log10(nnparticles))) + geom_tile() + scale_fill_viridis_c() + scale_y_reverse() + scale_x_log10() + geom_contour(color = "black") + geom_hline(yintercept = 160, color = "darkgreen")

Just 043

mbGam <- meanBese %>% group_by(depth)  %>% nest() %>%
  mutate(mod = map(data, ~gam(log(nnparticles) ~ log(lb), family = gaussian(), data = .))) %>% 
  mutate(psd = map_dbl(mod, ~summary(.)$p.coeff[2]))

mbGam %>% ggplot(aes(x = psd, y = depth)) + geom_path() + scale_y_reverse() + geom_hline(yintercept = 160, color = "darkgreen") + geom_hline(yintercept =  850, color = "darkblue")

mbGam <- meanBese043 %>% group_by(depth) %>% nest() %>% mutate(mod = map(data, ~gam(log(nnparticles) ~ log(lb), family = gaussian(), data = .))) %>% mutate(psd = map_dbl(mod, ~summary(.)$p.coeff[2]))

pWBPSD <- mbGam %>% ggplot(aes(x = psd, y = depth)) + geom_path() + scale_y_reverse()  + geom_hline(yintercept = 160, color = "darkgreen") + geom_hline(yintercept =  850, color = "darkblue")
pWBPSD

Fig 5 WB

bds %>% filter(profile == “stn_043”, depth <= 2000) %>% ggplot(aes(x = psd_gam, xmin = psd_gam - psd_seg * 2, xmax = psd_gam + psd_seg * 2, y = depth)) + geom_path(size = 1) + scale_y_reverse() + geom_hline(yintercept = 175, color = “darkgreen”) + geom_hline(yintercept = 950, color = “darkblue”) + geom_ribbon(alpha = 0.2) + labs(x = “PSD slope”)

All of them

bds %>% filter(profile == "stn_043", depth <= 2000) %>% ggplot(aes(x = psd_gam, xmin = psd_gam - psd_seg * 2, xmax = psd_gam + psd_seg * 2, y = depth)) + geom_path(size = 1) + scale_y_reverse() + geom_hline(yintercept = 175, color = "darkgreen") + geom_hline(yintercept = 950, color = "darkblue") + geom_ribbon(alpha = 0.2) + labs(x = "PSD slope")

043 only

bds %>% filter(profile == "stn_043", depth <= 2000, depth > 175) %>% ggplot(aes(x = small_biovolume, y = depth)) + geom_path(size = 1) + scale_y_reverse() + geom_hline(yintercept = 175, color = "darkgreen") + geom_hline(yintercept = 950, color = "darkblue") + geom_point()

ubDf0 <- ToPlot %>% mutate(ubiomass = nparticles * lb ^ ag_global)
ubDf <- ubDf0 %>% group_by(time, depth) %>% summarize(ubiomass = sum(ubiomass)) %>% ungroup %>% group_by(depth)
photicBiomass <- ubDf %>% filter(depth <= 180, depth >= 160) %>% summarize(ubiomass = mean(ubiomass)) %>% pull(ubiomass)
ubDf <- ubDf %>% mutate(nbiomass = ubiomass/photicBiomass)
ubDf %>% ggplot(aes(x = nbiomass, y = depth , group = time, col = time)) + geom_path() + scale_y_reverse() + scale_x_continuous(limits = c(0,1))

ubDf <- ToPlot %>% mutate(ubiomass = nparticles * lb ^ ag_global) %>% group_by(time, depth) %>% summarize(ubiomass = sum(ubiomass)) %>% ungroup %>% group_by(depth)  %>% summarise(ubiomass = mean(ubiomass))
photicBiomass <- ubDf %>% filter(depth <= 180, depth >= 160) %>% summarize(ubiomass = mean(ubiomass)) %>% pull(ubiomass)
ubDf <- ubDf %>% mutate(nbiomass = ubiomass/photicBiomass)
ubDf %>% ggplot(aes(x = nbiomass, y = depth)) + geom_path() + scale_y_reverse() + scale_x_continuous(limits = c(0,1)) + geom_hline(yintercept = 175, color = "darkgreen")

Small particles biomass

PubDf <- ToPlot %>% mutate(ubiomass = nparticles * lb ^ ag_global) %>% filter(lb < 0.5) %>% group_by(time, depth) %>% summarize(ubiomass = sum(ubiomass)) %>% ungroup %>% group_by(depth)  %>% summarise(ubiomass = mean(ubiomass))
photicBiomass <- PubDf %>% filter(depth <= 165, depth >= 155) %>% summarize(ubiomass = mean(ubiomass)) %>% pull(ubiomass)
PubDf <- PubDf %>% mutate(nbiomass = ubiomass/photicBiomass)
pWBS <- PubDf %>% ggplot(aes(x = nbiomass, y = depth)) + geom_path() + scale_y_reverse() + scale_x_continuous(limits = c(0,1.2)) + geom_hline(yintercept = 160, color = "darkgreen") + geom_vline(xintercept = 1, color = "gray50") + geom_vline(xintercept = 0, color = "gray50") + geom_hline(yintercept = 850, color = "darkblue") + labs( x = "Small particle mass (norm.)")
pWBS

LubDf <- ToPlot %>% mutate(ubiomass = nparticles * lb ^ ag_global) %>% filter(lb >= 0.5) %>% group_by(time, depth) %>% summarize(ubiomass = sum(ubiomass)) %>% ungroup %>% group_by(depth)  %>% summarise(ubiomass = mean(ubiomass))
photicBiomass <- LubDf %>% filter(depth <= 165, depth >=155) %>% summarize(ubiomass = mean(ubiomass)) %>% pull(ubiomass)
LubDf <- LubDf %>% mutate(nbiomass = ubiomass/photicBiomass)
pWBL <- LubDf %>% ggplot(aes(x = nbiomass, y = depth)) + geom_path() + scale_y_reverse() + scale_x_continuous(limits = c(0,1)) + geom_hline(yintercept = 160, color = "darkgreen") + labs( x = "Large particle mass (norm.)") + geom_vline(xintercept = 1, color = "gray50") + geom_vline(xintercept = 0, color = "gray50") + geom_hline(yintercept = 850, color = "darkblue")
pWBL

For tom and danielle

WBColorMap
pWBPSD
pWBS
pWBL

WBFig5 <- plot_grid(pWBPSD, pWBS,pWBL, nrow = 1, labels = c("B", "C", "D"))
WBFig5

WBcombined <- plot_grid(WBColorMap + theme(plot.margin = unit(c(0,3,0, 3), "cm")), WBFig5, ncol = 1, labels = c("A", ""))
WBcombined

ggsave("figures/WBModelValidation.png")

P16 Flux

scientific_10 <- function(x) {parse(text=gsub("e\\+*", " %*% 10^", scales::scientific_format()(x))) }
#https://stackoverflow.com/questions/10762287/how-can-i-format-axis-labels-with-exponents-with-ggplot2-and-scales
#jacob_magnitude <- function(x){expression(10^round(log10(x)))}

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
pltFlxP16 <- bds %>% filter(project == "P16") %>% #filter(DFP > 1) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = Flux_Smooth, group = factor(time)))  + geom_point(size = 3, stroke = 1)+
  geom_path() +
  scale_y_reverse(limits = c(1000, 0))+
  scale_x_log10(limits = c(35, 150),breaks = seq(from = 20, to = 150, by = 20)) +
   scale_color_gradient2(low = "darkgreen", mid = "gray80", high = "purple", midpoint = 10) + scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) + 
  scale_fill_gradientn(name = "Hour of Day", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  
labs(x = bquote(Smoothed~Flux~(µmol~C/m^2/d)), y = "Depth (m)") +
  geom_hline(yintercept = 200, color = "darkgreen") +
  theme(axis.text.x = element_text(angle = 90, vjust = .3), legend.spacing = unit(.1, "cm"))
# 
# 
# 
# pltFlxNoLegend <- pltFlx + theme(legend.position = "none")
# pltFlxLegend <- get_legend(pltFlx)
# 
pltFlxP16
# #plotly::ggplotly(plt1)

cb10 <- c('#a6cee3','#1f78b4','#b2df8a','#33a02c','#fb9a99','#e31a1c','#fdbf6f','#ff7f00','#cab2d6','#6a3d9a')
pltDelta3P16 <- bds %>% filter(project == "P16") %>% #filter(DFP > 1) %>% #filter(profile %in% c("stn_043", "p16n_100")) %>%
  ggplot(aes(y = depth, x = pracma::nthroot(DF/DZ, 5), group = factor(time)))  + geom_point(size = 3, stroke = 1)+
  geom_path() +
  scale_y_reverse(limits = c(1000, 0))+
  scale_x_continuous(limits = c(-1, .1), breaks = seq(from = -2, to = .75, by = 0.5)) +
  #scale_x_log10() +
   scale_color_gradient2(low = "darkgreen", mid = "gray80", high = "purple", midpoint = 10) + scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) + 
  scale_fill_gradientn(name = "Hour", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) +
  geom_vline(xintercept = 0) +
  geom_hline(yintercept = 200, color = "darkgreen")+
  labs(x = bquote((DF/DZ)^{1/5}~(µmolC/m^3/d)^{1/5}), y = "Depth (m)") + theme(legend.pos = "none")
  #labs(x = "(DF/DZ) ^ 1/5 (µmol C/m^3/d) ^ 1/5")

pltDelta3P16
#plotly::ggplotly(plt1pos)

osms_p16 <- bds %>% filter(project == "P16") %>%
  ggplot(aes(y = depth, x = pracma::nthroot(ospsDZ, 3), group = factor(time))) + geom_point(size = 3) + geom_path() + scale_y_reverse(limits = c(1000, 0)) +
  scale_x_continuous(limits = c(-1, 1)) +
  geom_vline(xintercept = 0) +   scale_shape_manual(name = "Day of Month", values = rep(21:25, 2)) + labs(x = "Observed - Modeled Small Particle Flux \n µmol/m^3/day") +
  scale_fill_gradientn(name = "Hour of Day", breaks = c(0, 6, 12, 18, 24), colors = c("black", "blue", "white", "orange", "black")) + geom_hline(yintercept = 175, color = "darkgreen") 
plotly::ggplotly(osms_p16)
#ggsave("..figures/FluxSizeShift.svg"

plot_grid(
  pltFlxP16,
  pltDelta3P16,
  osms_p16
)

ggsave("figures/P16FluxRelate.svg")
ggsave("figures/P16FluxRelate.png")

Flux attenuation example

source("ModelStuff.R")

scan_for_example <- bds %>% filter(project == "ETNP", depth < 500, depth > 200) %>% select(profile, depth, DFP, use_this_DFP, ospsDZ)

loc_station = "stn_036"
loc_depth = 225
loc_prev_depth = loc_depth - 50

loc_DFP <- bds %>% filter(profile == loc_station, depth %in% c(275)) %>% pull(DFP)
loc_use_DFP <- bds %>% filter(profile == loc_station, depth %in% c(275)) %>% pull(use_this_DFP)
for_single_disag <- bes %>% filter(profile == loc_station, depth %in% c(225, 275)) %>% select(depth, lb, nnp_smooth) %>%
  mutate(depth = recode(depth, `225` = "Shallow", `275` = "Deep")) %>%
  pivot_wider(names_from = depth, values_from = nnp_smooth) 

with_disag <- for_single_disag %>%
  mutate(Predicted_Deep = remin_smooth_shuffle(Shallow, loc_use_DFP)) 
#remin_smooth_shuffle(for_single_disag$Shallow,loc_use_DFP)

for_plot_disag <- with_disag %>% pivot_longer(cols = -lb) %>% filter(lb <= 0.5) %>%
  mutate(name = factor(name, levels = c("Shallow", "Deep", "Predicted_Deep"))) %>%
  mutate(name = recode_factor(name, Shallow = "Shallow (250m)", Deep = "Deep (275m)", Predicted_Deep = "Predicted Deep (275m)"))

for_plot_disag %>% ggplot(aes(x = lb, y = value, shape = name)) + geom_point() + scale_x_log10() + scale_y_log10() + scale_shape_manual(values = c(1, 6, 3)) + theme(legend.title = element_blank()) + labs(x = "Particle Size (mm)", y = "Normalized Particle Abundance (#/L/mm)")

ggsave("figures/DisagExample.png")
ggsave("figures/DisagExample.svg")

EK60

dataBinned <- read_csv("data/backscatter_table_go7.csv")

dataBinned_01 <- dataBinned %>%
  mutate(timeMex = with_tz(time_bin, tzone = "US/Central") )

startDay <- dataBinned_01$timeMex %>% na.omit %>% min %>% floor_date(unit = "days")
endDay <- dataBinned_01$timeMex %>% na.omit %>% max %>% ceiling_date(unit = "days")
timeBreaks <- seq(from = startDay, to = endDay, by = "12 hours")
timeLabels <- format(timeBreaks)

plotLetters <- tribble(
  ~letter, ~depth_bin, ~timeMex,
  "A", 350, as.POSIXct("2017-01-07 13:00:00"),
  "B", 200, as.POSIXct("2017-01-09 23:00:00"),
  "C", 150, as.POSIXct("2017-01-08 13:00:00"),
  "D", 625, as.POSIXct("2017-01-12 07:00:00"),
  "E", 750, as.POSIXct("2017-01-10 02:00:00")
  
)

library(shadowtext)
plot18k <- dataBinned_01 %>% filter(frequency == 18000) %>% ggplot(aes(x = timeMex, y = depth_bin, fill = value)) + geom_tile() + scale_y_reverse() + scale_fill_viridis_c(limits = c(-165, -75), oob = scales::squish) +
  scale_x_datetime(breaks = timeBreaks, date_labels = "%d::%H") + labs(x = "day::hour", y = "depth (m)", fill = "backscatter (dB)") + theme_bw() + theme(axis.text.x = element_text(angle = 90, vjust = 0.5)) + geom_hline(yintercept = 160, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") +
  geom_shadowtext(data = plotLetters, aes(x = timeMex - 12 * 60^2, y = depth_bin, label = letter),  inherit.aes = FALSE, size = 6, bg.color = "white", color = "black") +
  geom_segment(data = plotLetters, inherit.aes = FALSE, aes(x = timeMex - 9 * 60^2, xend = timeMex, y = depth_bin, yend = depth_bin), arrow = arrow(length = unit(0.03, "npc")), color = "white", size = 1.5) + 
  geom_segment(data = plotLetters, inherit.aes = FALSE, aes(x = timeMex - 9 * 60^2, xend = timeMex, y = depth_bin, yend = depth_bin), arrow = arrow(length = unit(0.03, "npc")))
plot18k

#ggsave("figures/stationP2_EK60_18kOnly.png")

plotLetters <- tribble(
  ~letter, ~depth_bin, ~timeMex,
  "A", 300, as.POSIXct("2017-01-07 13:00:00"),
  "B", 200, as.POSIXct("2017-01-09 23:00:00"),
  "C", 150, as.POSIXct("2017-01-08 13:00:00"),
  "D", 625, as.POSIXct("2017-01-12 07:00:00"),
  "E", 750, as.POSIXct("2017-01-10 02:00:00")
  
)

library(shadowtext)
plot38k <- dataBinned_01 %>% filter(frequency == 38000) %>% ggplot(aes(x = timeMex, y = depth_bin, fill = value)) + geom_tile() + scale_y_reverse() + scale_fill_viridis_c(limits = c(-165, -95), oob = scales::squish) +
  scale_x_datetime(breaks = timeBreaks, date_labels = "%d::%H") + labs(x = "Day::Hour", y = "Depth (m)", fill = "Backscatter (dB)") + theme_bw()  + geom_hline(yintercept = 160, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue") +
  geom_shadowtext(data = plotLetters[1:2,], aes(x = timeMex - 12 * 60^2, y = depth_bin, label = letter),  inherit.aes = FALSE, size = 6, bg.color = "white", color = "black") +
  geom_segment(data = plotLetters[1:2,], inherit.aes = FALSE, aes(x = timeMex - 9 * 60^2, xend = timeMex, y = depth_bin, yend = depth_bin), arrow = arrow(length = unit(0.03, "npc")), color = "white", size = 1.5) + 
  geom_segment(data = plotLetters[1:2,], inherit.aes = FALSE, aes(x = timeMex - 9 * 60^2, xend = timeMex, y = depth_bin, yend = depth_bin), arrow = arrow(length = unit(0.03, "npc"))) +
  theme(axis.text.x = element_text(size = 12,angle = 90, vjust = 0.5),
        axis.text.y = element_text(size = 12),
        axis.title = element_text(size = 14),
        legend.title = element_text(size = 14)
        )
plot38k

ggsave("figures/stationP2_EK60_38kOnly.png")

plot18k + scale_y_reverse(limits = c(500, 200))

plot200k <- dataBinned_01 %>% filter(frequency == 200000) %>% ggplot(aes(x = timeMex, y = depth_bin, fill = value)) + geom_tile() + scale_y_reverse() + scale_fill_viridis_c(limits = c(-165, -155), oob = scales::squish) +
  scale_x_datetime(breaks = timeBreaks, date_labels = "%d::%H") + labs(x = "day::hour", y = "depth (m)", fill = "backscatter (dB)") + theme_bw() + theme(axis.text.x = element_text(angle = 90, vjust = 0.5)) + geom_hline(yintercept = 160, color = "darkgreen") +
  geom_hline(yintercept = 850, color = "darkblue")
plot200k

dataBinned_01 %>% ggplot(aes(x = timeMex, y = depth_bin, fill = value)) + geom_tile() + scale_y_reverse() + scale_fill_viridis_c(limits = c(-165, -75), oob = scales::squish) +
  scale_x_datetime(breaks = timeBreaks, date_labels = "%d::%H") + labs(x = "day::hour", y = "Depth (m)", fill = "backscatter (dB)") + theme_bw() + theme(axis.text.x = element_text(angle = 90, vjust = 0.5)) +
  
  
  facet_wrap(~frequency, ncol = 1)

#ggsave("figures/stationP2_EK60_go7.svg", width = 4, height = 10)
ggsave("figures/stationP2_EK60_go7.png", width = 6, height = 10)